![]() Store the activated stain at room temperature. Dissolve the crystals by mixing end-over-end for one minute, then allow it to remain at room temperature for five minutes before use. Allow buffer to drain from column into tube. 8.2 (Optional) Desalt HIC fractions using the P6 Spin Columns: 8.2.1 After resuspending the gel in P6, snap off the tip of the column and place it in a 2.0 ml microfuge tube. 8.1.6 GFP standard diluted 5 x, then suspended in 2X laemmli buffer. Add the entire contents of the activator crystals bottle into the GelCode Blue Safe Protein Stain bottle. 8.1.5 Kaleidoscope MW protein ladder, 10 l. Time-Saving Tip: GelCode Blue can be left on the gel overnight. For proper protein staining, the activator crystals must be dissolved in the staining reagent before use. The molecular weight marker does not need SDSPAGE sample buffer added to it. ![]() Department of Transportation (DOT) shipping requirements.
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